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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Plasma proteomic analysis of autoimmune hepatitis in an improved AIH mouse model

Fig. 4

Autoantibody production and immune cell infiltration in the AIH mouse model. a Representative pictures of rat liver sections stained with plasma from AIH mice (primary antibody) at day 35, followed by Alexa Fluor 488-conjugated anti-mouse IgG (secondary antibody). The plasma from AIH-1 (type-1 autoimmune hepatitis) patients (antinuclear antibodies, ANAs), AIH-2 (type-2 autoimmune hepatitis) patients (anti-liver-kidney microsomal-1 (LKM-1) antibodies), and PBC patients (anti-mitochondrial antibodies, AMAs) were used to stain the same rat liver sections as the primary antibodies. FITC-conjugated anti-human IgG was used as the secondary antibody (n = 3, upper column: ×200 magnification, lower column: ×800 magnification). b The dilution ratio of autoantibodies in the plasma of AIH mouse after the first injection (n = 7). c CD4+ T cells and CD8+T cells were found to accumulate in the AIH mouse liver on different days after the first injection, with CD4 (red) and CD8 (green) as their marker respectively. Nuclei were stained with Hoechst (blue) (n = 5, ×600 magnification). **P < 0.01, ***P < 0.001 vs. the control group

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