Fig. 2

Chronic liver inflammation and pathological features of autoimmune hepatitis in the mouse model. a Real-time PCR was used to detect the transfection efficiency of the CYP2D6 plasmid in the mouse livers (n = 6, ***P < 0.001 vs. Control). b The expression of CYP2D6 protein in the mouse liver was detected by western blotting (n = 3; P1–3 indicates the mice injected with CYP2D6 plasmid and C1–3 indicates control mice injected with PBS). c C57 BL/6 mice were sacrificed at day 14, day 28, day 36, and day 42 post the first injection. The liver tissue was harvested and analyzed by H&E staining (n = 6, ×200 and ×400 magnification). Only one control group which injected PBS was shown in this figure. Other control groups (only injected with adenovirus or plasmid) were shown in Additional file 3: Figure S2. d Liver inflammatory score of the AIH mouse model at different days (based on the Knodell histological activity index, HAI, a hepatitis scoring system). e Interfacial hepatitis was observed in the mouse livers at day 36, and rosettes and lymphocytes invasion were also detected (f); the arrows indicate the lymphocytes