Skip to main content
Fig. 6 | Journal of Translational Medicine

Fig. 6

From: ROR2 induces cell apoptosis via activating IRE1α/JNK/CHOP pathway in high-grade serous ovarian carcinoma in vitro and in vivo

Fig. 6

Kira6 reversed the apoptosis and activation of IRE1α/JNK/CHOP pathway induced by ROR2 overexpression. a HEY and HO-8910 cells were treated with Kira6 for 72 h after pre-transfected with ROR2 overexpression or negative control adenovirus for 6 h. Apoptosis was detected by flow cytometry after staining with FITC Annexin-V and PI. Quantitive analysis of apoptotic ratio in HEY and HO-8910 cells with CellQuest Pro software. Statistical analysis was performed with GraphPad Prism using Student’s t test. b HEY and HO-8910 cells were treated with Kira6 for 72 h after pre-transfected with ROR2 overexpression or negative control adenovirus for 6 h. Expression of ROR2, phosphorylated IRE1α, CHOP, phosphorylated JNK, phosphorylated c-Jun, Bax, Bcl-2, cleaved caspase3, cleaved caspase7, and cleaved PARP were determined by western-blot assay. β‑actin was used as a loading control. c Quantitation of western-blot assay bands shown in b using Image J. Statistical analysis was performed using Student’s t test. p-IRE1α phosphorylated IRE1α (Ser724), p-JNK phosphorylated JNK (Thr183/Tyr185), p-c-Jun phosphorylated c-Jun (Ser73). All experiments were repeated three times at least. *P < 0.05, **P < 0.01 and ***P < 0.001 for statistical analysis of the indicated groups

Back to article page