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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: ROR2 induces cell apoptosis via activating IRE1α/JNK/CHOP pathway in high-grade serous ovarian carcinoma in vitro and in vivo

Fig. 5

IRE1α knockdown reversed the apoptosis and activation of IRE1α/JNK/CHOP pathway induced by ROR2 overexpression. a Expression of IRE1α in HEY and HO-8910 cells were determined by western-blot assay after transfected with si-IER1α or si-NC for 72 h. β‑actin was used as a loading control. b HEY and HO-8910 cells were transfected with ROR2 or NC adenovirus for 48 h after pre-transfected with si-IRE1α for 24 h. Apoptosis was detected by flow cytometry after staining with FITC Annexin-V and PI. Quantitive analysis of apoptotic ratio in HEY and HO-8910 cells with CellQuest Pro software. Statistical analysis was performed with GraphPad Prism using Student’s t test. c HEY and HO-8910 cells were transfected with ROR2 or NC adenovirus for 48 h after pre-transfected with si-IRE1α for 24 h. Expression of ROR2, IRE1α, phosphorylated IRE1α, CHOP, phosphorylated JNK, phosphorylated c-Jun, Bax, Bcl-2, cleaved caspase3, cleaved caspase7, and cleaved PARP were determined by western-blot assay. β‑actin was used as a loading control. d Quantitation of western-blot assay bands shown in c using Image J. Statistical analysis was performed using Student’s t test. NC negative control, p-IRE1α phosphorylated IRE1α (Ser724), p-JNK phosphorylated JNK (Thr183/Tyr185), p-c-Jun phosphorylated c-Jun (Ser73). All experiments were repeated three times at least. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P< 0.0001 for statistical analysis of the indicated groups

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