Fig. 4

ROR2 overexpression activated IRE1α/CHOP/JNK pathway. a Gene ontology analysis was performed in ROR2-overexpressed HO-8910 cells compared to negative control cells. Top ten enriched biological processes, cell components and molecular functions was shown. b Cells were transfected with ROR2 overexpression or negative control adenovirus for 72 h. Expression of ROR2, IRE1α, phosphorylated IRE1α, CHOP, JNK, phosphorylated JNK, c-Jun, phosphorylated c-Jun was determined by western-blot assay. β‑actin was used as a loading control. c Quantitation of wester-blot assay bands shown in b using Image J. Statistical analysis was performed using Student’s t test. NC negative control, p-IRE1α phosphorylated IRE1α (Ser724), p-JNK phosphorylated JNK (Thr183/Tyr185), p-c-Jun phosphorylated c-Jun (Ser73). All experiments were repeated three times at least. *P < 0.05, **P < 0.01 and ***P < 0.001 for statistical analysis of the indicated groups