Fig. 1

ROR2 was downregulated in HGSOC tissues and its expression was correlated with HGSOC FIGO stages. a Western-blot assay was used to detect the expression of ROR2 in 23 HGSOC tissues and 23 normal fallopian tube tissues. b Quantitation of western-blot assay bands shown in a using Image J. Statistical analysis was performed using Student’s t test. c Validation of ROR2 expression from 3 GEO databases, GSE69428, GSE40595 and GSE18520. The mRNA level of ROR2 was analyzed with the “limma” package using Student’s t test. d Immunohistochemical staining was used to detect the expression of ROR2 in 81 HGSOC tissue samples. e The intensities of IHC staining were quantitated by Image-Pro Plus 6.0. ROR2 IHC scores in different FIGO stages were analyzed with Mann–Whitney test. f The intensities of IHC staining were quantitated by Image-Pro Plus 6.0. ROR2 IHC scores in patients with different lymph nodes status were analyzed with Mann–Whitney test. LN−: patients with negative lymph nodes. LN+: patients with positive lymph nodes. *P < 0.05, **P < 0.01, and ***P < 0.001 for statistical analysis of the indicated groups