Fig. 3

The effects of FK506 on the mRNA and protein expression of FXR and related glycometabolic genes. a, b FK506 (15 µM) inhibited the protein expression of FXR at 24, 48 and 72 h in the HK-2 cell lines. The mRNA levels of FXR (c), SHP1 (d) and GLUT2 (f) were clearly downregulated by FK506 at the same time. e The mRNA level of PEPCK was obviously upregulated by FK506 treatment for 24, 48 and 72 h. Data are presented as the mean ± SD (n = 3 per group). *P < 0.05 vs. the control group