Fig. 8

Impacts of Gdf11 gene transfer on expression of genes involved in inflammation, thermogenesis, and glucose and lipid metabolism. Tissue was isolated from mice at the end of 8-week feeding period after gene transfer. a Relative mRNA levels of inflammatory genes and macrophage markers in the EWAT; b histoimmunochemistry of WAT with F4/80 antibody; c relative mRNA levels of thermogenic genes in brown adipose tissue; d the protein level of UCP1 and UCP2 in BAT (lift side) and scanning density of the protein bands (right side); e relative mRNA levels of genes involved in lipid metabolism in the liver; f relative mRNA levels of genes involved in β oxidation in the liver; g relative mRNA levels of genes involved in glucose metabolism in the liver. Each data point represents mean ± SEM (n = 5). *P < 0.05 comparing to mice fed regular Chow, ^#P < 0.05 comparing to mice fed an HFD and injected with control plasmids