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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Does HOTAIR expression level in the peripheral blood have veritably predictive/prognostic impact on breast cancer patients?

Fig. 1

Schematic representation of the quantitative amplification plot and melt curve for aGAPDH molecule in cDNA samples, b HOTAIR molecule in cDNA samples and cHOTAIR molecule in genomic DNA samples. Image d also schematically shows qualitative fragment analyses of the indicated PCR products. The samples 1, 2 and 3 are respectively representative images of normal individual, breast cancer patients before and after radiotherapy (10 Gy). A specific fragment was determined for GAPDH (146 bp), while no fragment was generated from HOTAIR amplification in cDNA samples (potential cDNA fragment length: 197–201 bp depending on the different HOTAIR transcript variants; potential genomic fragment length: 389 bp). A specific fragment was also detected for HOTAIR (389 bp) in genomic DNA of all representative samples. NTC; Non-template control

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