Fig. 1

BZRAP1-AS1 is highly expressed in HCC tissues and cells. a, b Differentially expressed lncRNAs screened from HCC-related microarrays GSE33006 and GSE49515. The abscissa shows the number of sample and the ordinate shows differentially expressed genes. The histogram in the upper right is the color gradation, and each rectangle in the figure corresponds to the expression of one sample. c Expression of BZRAP1-AS1 relative to GAPDH in HCC tissues and the adjacent normal tissues. d Expression of BZRAP1-AS1 relative to GAPDH in HCC cells and the normal liver cell line. e Localization of BZRAP1-AS1 in HCC cells analyzed by lncATLAS online site. f Subcellular localization (×200) of BZRAP1-AS1 in HuH-7 cells detected by FISH. g Nuclear expression of BZRAP1-AS1 in HuH-7 cells determined by RT-qPCR. *p < 0.05, vs. adjacent normal tissues; ^#p < 0.05 vs. L-02 cell line. The data (mean ± S.D.) obeying the normal distribution variance between two groups were analyzed using unpaired t test while those among multiple groups were assessed by one-way ANOVA, followed with Tukey’s post hoc test. The experiment was repeated 3 times independently. BZRAP1-AS1 BZRAP1-antisense RNA 1, HCC hepatocellular carcinoma, FISH fluorescence in situ hybridization, ANOVA analysis of variance