The impact of cryopreservation on bone marrow-derived mesenchymal stem cells: a systematic review

Table 8 Bone-marrow derived Mesenchymal Stem Cell studies evaluating cellular attachment post-thaw

Study	Species	Results post-thaw	Method of assessment
Attachment
Human
Heng [30]	Human	Level of adherent cells was 39.8 ± 0.9%; increased by approx. 10% with Y-27632	MTT assay performed 24 h post-thawing
Chinnadurai et al. [20]	Human	40% reduction in adhesion to fibronectin; 80% reduction in adhesion to endothelial cells; No reduction in the surface expression of adhesion molecules	After 2 h in static and 1 h in vascular flow conditions using microscopy (light); Flow cytometry for adhesion molecules
Other species
Li et al. [96]	Dog	Decreased adhesion capacity post-thaw; recovery of adhesion capacity after culturing for several passages	Adherent cell count (hemo-cytometer) at 4, 8, 12 and 24 h post-thaw
Tokumoto et al. [48]	Monkey	Limited influence of cryopreservation on cell adhesion capabilities	Adherent cell count (hemo-cytometer)
Migration
Human
Yuan et al. [52]	Human (BM-MSC engineered to express TRAIL)	No effect on migration potential	Trans-well plates

The effects of cryopreservation on bone-marrow derived mesenchymal stem cell attachment are presented in this table. For further details on the cryopreservation experimental details refer to either Table 1 or Additional file 2 which provide the individual freezing protocols outlined in the extracted papers alongside the concentration and passage of cells at the point of cryopreservation and the process of thawing

ISSN: 1479-5876