From: The impact of cryopreservation on bone marrow-derived mesenchymal stem cells: a systematic review
Study | Species | Results post-thaw | Method of assessment |
---|---|---|---|
Human | |||
 Liu et al. [28] | Human | Reduced-DMSO freezing solution gives comparable metabolic activity to 10% DMSO | AlamarBlue assay |
 Chinnadurai et al. [20] | Human | No reduction in metabolic fitness | Calcium uptake; PrestoBlue reduction |
 Chinnadurai et al. [68] | Human | The addition of various concentrations of hPL (human platelet lysate) did not significantly enhance MSC metabolic activity | PrestoBlue reduction |
Other species | |||
 Liu et al. [29] | Rat, mouse and calf | In general, non-cryopreserved cells showed higher overall metabolic activities than the cryopreserved; Reduced DMSO (5%) with 2% PEG, 3% trehalose and 2% albumin give superior results to 10% DMSO | AlamarBlue assay |
 Nitsch et al. [97] | Monkey | Lower metabolic activity for cryopreserved cells compared with fresh; Enhnaced levels of metabolic activity obtained for 5% and 10% DSMO levels | MTT assay (24 h, 48, 72 and 96 h post-thaw) |
 Lauterboeck et al. [49] | Monkey | Cells’ metabolic activity was impaired up until 48 h post-thaw; Partial recovery at 72 h and full recovery observed at 96 h | MTT assay (24 h, 48, 72 and 96 h post-thaw) |