From: The impact of cryopreservation on bone marrow-derived mesenchymal stem cells: a systematic review
Study | Species | Results post-thaw | Method of assessment |
---|---|---|---|
Human | |||
 Bruder et al. [91] | Human | No effect on proliferation | Cell re-plated for one passage post-thaw; crystal violet dye-binding method |
 Haack-Sorensen et al. [19] | Human | No effect on proliferation | PKH26-GL cell linker kit |
 Xiang et al. [93] | Human | No effect on proliferation | Growth curves |
 Zhao et al. [94] | Human (with chronic myeloid leukaemia) | No effect on proliferation | Cell count and cell-doubling time |
 Doan et al. [95] | Human | No effect on proliferation | NA |
 Ginis et al. [50] | Human | Proliferation of cryopreserved cells after 1 or 5 months of storage was higher than non-cryopreserved cells | Calcein-AM staining (at day 1, 4, 7 and 14 after post-thaw plating) |
 Mamidi et al. [33] | Human | No effect on proliferation | Population doublings, cumulative population doublings and population doubling time |
 Matsumura et al. [26] | Human | No effect on proliferation | Cell count; population doubling time (24 h, 48 h, 72 h and 96 h post-thaw) |
 Holubova et al. [69] | Human | No effect on proliferation | Cell count |
 Al-Saqi et al. [66] | Human | No significant difference in population doubling time but cells cryopreserved in DMSO had longer population doubling time compared to fresh | Population doubling (first and second passage post-thaw) |
 Luetzkendorf et al. [40] | Human | No effect on proliferation | Population doublings; Population doubling time |
 Pollock et al. [67] | Human | Population doublings decreased with increasing pre-freeze passage number | Population doublings |
 Lechanteur et al. [34] | Human | Very low recovery until day 4 then a slight increase indicating re-proliferation | Cell count (0–5 days after thawing) |
 Yuan et al. [52] | Human (BM-MSC engineered to express TRAIL) | No effect on proliferation | XTT assay |
Other species | |||
 Edamura et al. [36] | Dog | DMSO and FBS-free freezing resulted in similar proliferative capacity as non-cryopreserved; DMSO and FBS containing freezing media gave lower proliferative capacity | Cell count (2, 4, 6,8, 10 and 12 days post-thaw) |
 Tokumoto et al. [48] | Monkey | No effect on proliferation | DNA quantification at 4, 8 and 12 days |
 Lauterboeck et al. [49] | Monkey | No effect on proliferation | Population doubling time |
 Heino et al. [39] | Minipig | Two to sixfolds decrease in the proliferative capacity of cells | Population doublings |
 Romanek et al. [98] | Pig (BM-MSC treated with a high hydrostatic pressure (HHP) before freezing) | Cells treated with HHP showed better proliferation rate | Cell count |
 Mitchell et al. [32] | Horse | No effect on proliferation | Cell staining with CellTrace label |
Colony-forming unit ability | |||
 Human | |||
  Verdanova et al. [25] | Human | Best number of colonies obtained when cells were frozen with 5% DMSO with 5% sericin in culture medium | Cells seeded 60 cm Petri dishes for 2 weeks, Crystal Violet stained and colonies counted (light microscope) |
 Other species | |||
  Ock and Rho, [51] | Pig | All cryopreserved cells showed significantly lower numbers of colonies compared to fresh; Lower DMSO produced higher number of colonies | Cells seeded in 6-well plates for 2 weeks, 4% Giemsa stained and colonies counted (light microscope) |
  Mitchell et al. [32] | Horse | No effect on colony-forming unit ability | Cells seeded in 10 cm plates for 1 week, Crystal Violet stained and colonies counted (light microscope) |