Table 3 Bone-marrow derived Mesenchymal Stem Cell studies evaluating surface marker expression post-thaw
From: The impact of cryopreservation on bone marrow-derived mesenchymal stem cells: a systematic review
Study | Species | Results post-thaw | Method of assessment |
|---|---|---|---|
Human | |||
 Kotobuki et al. [92] | Human | No difference | Flow cytometry |
 Haack-Sorensen et al. [19] | Human | No difference | Flow cytometry |
 Xiang et al. [93] | Human | No difference | Fluorescent sorting at passage 1, 5, 10 and 15 post-thaw |
 Zhao et al. [94] | Human (with chronic myeloid leukaemia) | No difference | Flow cytometry |
 Doan et al. [95] | Human | No difference | Flow cytometry |
 Ginis et al. [50] | Human | No difference except lower expression of CD9 | Flow cytometry |
 Mamidi et al. [33] | Human | No difference | Flow cytometry |
 Matsumura et al. [26] | Human | No difference | Flow cytometry |
 Holubova et al. [69] | Human | No difference | Flow cytometry |
 Moll et al. [38] | Human | No difference | Flow cytometry |
 Al-Saqi et al. [66] | Human | No difference | Flow cytometry |
 Luetzkendorf et al. [40] | Human | No difference | Flow cytometry |
 Yuan et al. [52] | Human (BM-MSC engineered to express TRAIL) | No difference | Flow cytometry |
Other species | |||
 Naaldijk et al. [27] | Rat | No difference | Flow cytometry |
 Davies et al. [42] | Rat | No change in the expression of CD29 and CD73; Increase in the expression of CD90, CD44 and CD105 | Flow cytometry for CD29 and CD90; RT-qPCR for CD44, CD105 and CD73 |
 Ock and Rho [51] | Pig | No difference | Flow cytometry |