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Table 2 Bone-marrow derived mesenchymal stem cell studies assessing post-thaw cell morphology

From: The impact of cryopreservation on bone marrow-derived mesenchymal stem cells: a systematic review

Study

Species

Results post-thaw

Method of assessment

Human

 Kotobuki et al. [92]

Human

No effect on morphology

Microscopy (fluorescent/phase contrast)

 Haack-Sorensen et al. [19]

Human

No effect on morphology

NA

 Xiang et al. [93]

Human

No effect on morphology

Microscopy (light) at cell confluency post-thaw

 Zhao et al. [94]

BM-MSC (human with chronic myeloid leukemia)

No effect on morphology

NA

 Heng [30]

Human

The addition of Y-27632 altered the morphology of the cells (web-like appearance)

NA

 Liu et al. [28]

Human

No effect on morphology

Microscopy (fluorescent)

 Doan et al. [95]

Human

No effect on morphology

Microscopy (light) 7 days post

 Mamidi et al. [33]

Human

No effect on morphology

NA

 Moll et al. [38]

Human

Effect of cryopreservation seen on forward scatter but not side scatter when exposed to human serum

Microscopy; cell counter and analyser system (CASY-TT); Flow cytometry

 Al-Saqi et al. [66]

Human

No effect on morphology

Microscopy (light) two passages post

Other species

 Liu et al. [29]

Rat, mouse and calf

No effect on morphology

Microscopy (light)

 Naaldijk et al. [27]

Rat

No effect on morphology

Microscopy (light)

 Davies et al. [42]

Rat

No effect on morphology

Microscopy (phase contrast)

 Zhu et al. [46]

Dog

Cells had several shapes such as long fusiform, polygon and astroid

Checked at days 2 and 5 after thawing NA

 Edamura et al. [36]

Dog

No effect on morphology

Microscopy (light)

 Mitchell et al. [32]

Horse

No effect on morphology

Microscopy (light) (24 and 72 h post)

  1. The key results on bone-marrow derived mesenchymal stem cell morphology are presented in this table. For further details on the cryopreservation experimental details refer to either Table 1 or Additional file 2 which provide the individual freezing protocols outlined in the extracted papers alongside the concentration and passage of cells at the point of cryopreservation and the process of thawing