From: The impact of cryopreservation on bone marrow-derived mesenchymal stem cells: a systematic review
Study | Species | Results post-thaw | Method of assessment |
---|---|---|---|
Human | |||
 Kotobuki et al. [92] | Human | No effect on morphology | Microscopy (fluorescent/phase contrast) |
 Haack-Sorensen et al. [19] | Human | No effect on morphology | NA |
 Xiang et al. [93] | Human | No effect on morphology | Microscopy (light) at cell confluency post-thaw |
 Zhao et al. [94] | BM-MSC (human with chronic myeloid leukemia) | No effect on morphology | NA |
 Heng [30] | Human | The addition of Y-27632 altered the morphology of the cells (web-like appearance) | NA |
 Liu et al. [28] | Human | No effect on morphology | Microscopy (fluorescent) |
 Doan et al. [95] | Human | No effect on morphology | Microscopy (light) 7 days post |
 Mamidi et al. [33] | Human | No effect on morphology | NA |
 Moll et al. [38] | Human | Effect of cryopreservation seen on forward scatter but not side scatter when exposed to human serum | Microscopy; cell counter and analyser system (CASY-TT); Flow cytometry |
 Al-Saqi et al. [66] | Human | No effect on morphology | Microscopy (light) two passages post |
Other species | |||
 Liu et al. [29] | Rat, mouse and calf | No effect on morphology | Microscopy (light) |
 Naaldijk et al. [27] | Rat | No effect on morphology | Microscopy (light) |
 Davies et al. [42] | Rat | No effect on morphology | Microscopy (phase contrast) |
 Zhu et al. [46] | Dog | Cells had several shapes such as long fusiform, polygon and astroid | Checked at days 2 and 5 after thawing NA |
 Edamura et al. [36] | Dog | No effect on morphology | Microscopy (light) |
 Mitchell et al. [32] | Horse | No effect on morphology | Microscopy (light) (24 and 72 h post) |