Fig. 6

MTX-treated animals present with a lower plaque burden and lower relative YAP/TAZ expression levels in DF regions. a, b Representative H&E and Masson-stained normal left carotid artery (n = 3) sections from the upstream region of the cast (0.5 mm proximal from the cast) where DF was induced after 0.9% saline treatment for 4 weeks (n = 7) and sections after treatment with MTX (n = 8). c Representative immunofluorescence image of the normal left carotid artery treated with 0.9% saline or MTX. Sections were stained with YAP/TAZ (red) and DAPI (Blue). Sections are stained with anti-CD31 as an endothelial marker (green). MTX-treated animals showed lower relative YAP/TAZ expression levels in DF regions