Fig. 5

MTX has no effect on DF-induced HUVEC apoptosis and atorvastatin promotes YAP phosphorylation. a HUVECs were incubated with MTX for 48 h and subjected to biomechanical stretch for another 10 h. Apoptosis in HUVECs is shown as flow cytometry dot plots. b As quantified by flow cytometry, DF increased the total apoptosis rate compared to that under static (STA) conditions. However, treatment with MTX did not reduce apoptosis. The percentage of apoptotic cells is presented as the mean ± SD from triplicate experiments. ****p < 0.0001 vs. STA. c Western blotting results (left panel) and quantitative data (right panel) for the protein levels of p-YAP are presented. Values are presented as mean ± SD from three independent experiments. **p < 0.01, ***p < 0.001 vs. DF