Fig. 6From: Comparison and characterization of enriched mesenchymal stem cells obtained by the repeated filtration of autologous bone marrow through porous biomaterialsComparison of MSC morphology and osteogenetic differentiation fate before and after filtration. A, B Primary bone marrow MSCs were cultured for 10 days before (A) and after filtration (B), and the cell morphology was observed on day 10; C, D Alkaline phosphatase staining of pre- (C) and postfiltration (D) MSCs and their quantitative analysis (J). E, F Alizarin red staining of pre- (E) and postfiltration (F) MSCs and their quantitative analysis (K). G, H The osteogenetic marker OPN was stained green in pre- (G) and postfiltration (H) MSCs. I No significant difference was noted in the MSC aspect ratio before and after filtration; OPN osteopontinBack to article page