Fig. 5

Hierarchical clustering analysis of the expression of 10 genes predictive of single BMSC time in culture. The binary classification algorithm, based on the mean gene expression for each cluster, was used for gene expression rankings to generate marker predictions which were assessed with receiver operating characteristic (ROC) curves and Wilcoxon signed rank test for p-values. The resulting 10 genes that meet marker gene definitions of area under the ROC curve (AUROC) > 0.85 and p-value < 0.01 are shown. The expressions of these 10 genes among single BMSCs from passages 3, 4, 6, 8 and 9 were analyzed by hierarchical clustering analysis