Fig. 2

Expression of stromal cell marker genes by BMSCs as measured by RNA-Sequencing. The transcriptome of BMSCs from passages 2, 4, 6, 9 and 10 was analyzed by RNA-Seq. The expression of stromal cell markers CD73, CD90, CD105, CD146 and CD166 are shown in a and the expression of hematopoietic cell markers in CD45, CD34, CD14 and CD11b in b. The expression of four genes differentially expressed among early and late passage cells is shown in c. Among the genes whose expression was greatest in early passage BMSC, the fold difference was greatest for CRISPLD2, and VCAM1 is a functionally important gene. Among the genes whose expression was greatest in late passage BMSCs, the fold difference was greatest for ESM1, and CXCL8 is a functionally important gene