Fig. 6

Microvascular remodeling in the kidney graft exposed to high-fat diet. Representative renal α-SMA staining in kidney grafts 3 months after reperfusion in normal (A-a) or high-fat diet (A-b) groups (up, A) and quantification of media-to-lumen ratio (10 vessels by field, down, A). We explored in the same groups tubular atrophy by HES staining (Magnif. ×40; B); and insterstitial fibrosis by Masson trichrome staining (Magnific. ×40; C; n = 5 in each group). Scale bars represent 100 µm. Representative three-dimensional tomographic images of the renal cortical microcirculation in normal diet (D-a) or in hyperlipidic diet (D-b) and spatial density from different size vascular segments (D-c). High-fat diet reduced the average of vascular segment diameter (D-d; n = 5 in each group). Values significantly different from the normal diet group are represented by *p < 0.05