Fig. 5

Additional stimulation of whole blood with with C3a and C5a does not affect IL2 release assay sensitivity. IL2 responses to HBsAg were assessed in presence of a, b C3a, c, d C5a as well as e, f C3a and C5a. Shown are for each condition a, c, e receiver-operating-characteristic (ROC) curves including calculated AUC (area under the curve) as well as b, d, f scatter plot of IL2 release of HepB vaccinated and unvaccinated HC after stimulation of whole blood. All depicted values were corrected by the negative control. Dotted line indicates IL2 cut-off at b 10.4 pg/ml (n = 78; n = 27 anti-HBs positive and n = 51 anti-HBs negative), d 71.7 pg/ml (n = 74; n = 22 anti-HBs positive and n = 52 anti-HBs negative), f 55 pg/ml (n = 55; n = 17 anti-HBs negative and n = 38 anti-HBs positive)