Fig. 2

C5a specifically enhances IFNγ and IL2 responses to HBsAg in whole blood of healthy donors. a IFNγ and b IL2 responses in whole blood following stimulation with HBsAg, HBsAg and C3a, HBsAg and C5a as well as HBsAg, C3a and C5a. All depicted values were corrected by the negative control and are given as mean concentration pg/ml ± SEM. Lower limit of detection (Background + 3 × SD) was at 1.9–12.6 pg/ml for IFNγ and at 2.6–9.8 pg/ml for IL2. Shapiro–Wilk normality test followed by Friedman test. Following symbols pinpoint significant differences: *, ^§One symbol equals p < 0.05, two symbols p < 0.01, three symbols p < 0.001 (n = 55)