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Table 3 IFA staining analysis for AP-1 transcription factors in GBM cell lines

From: Identification of a novel role of IL-13Rα2 in human Glioblastoma multiforme: interleukin-13 mediates signal transduction through AP-1 pathway

AP-1 transcription factors {extent of immunostaining (% positive cells)}
  IL13Rα2 c-Jun Fra-1 JunD c-Fos JunB
Control +IL13 Control +IL13 Control +IL13 Control +IL13 Control +IL13 Control +IL13
U251 3 + 3 + (> 70%)* 1.5+ 3 + (> 70%)* 1 + 3.5 + (> 70%)* 1 + 2 + (< 50%)* 1 + 2 + (< 20%)* < 1 < 1 (0%)*
A172 3 + 3 + (> 55%)* < 1 2 + (40%)* < 1 2 + (> 50%)* < 1 1 + (< 30%)* < 1 <1 (< 10%)* < 1 < 1 (0%)*
T98G < 1 < 1 (0%)* < 1 < 1 (0%)* < 1 <1 (0%)* < 1 <1 (0%)* < 1 <1 (0%)* < 1 < 1 (0%)*
  1. GBM cell lines were immunostained for extent of immunostaining for each phenotype. ≤ 1+, negative; 2 + , positive; 3 + , strongly positive; 4 + , more strongly positive. % cells are positive stained cells counted per total 100 cells (*)