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Table 3 IFA staining analysis for AP-1 transcription factors in GBM cell lines

From: Identification of a novel role of IL-13Rα2 in human Glioblastoma multiforme: interleukin-13 mediates signal transduction through AP-1 pathway

AP-1 transcription factors {extent of immunostaining (% positive cells)}

 

IL13Rα2

c-Jun

Fra-1

JunD

c-Fos

JunB

Control

+IL13

Control

+IL13

Control

+IL13

Control

+IL13

Control

+IL13

Control

+IL13

U251

3 +

3 + (> 70%)*

1.5+

3 + (> 70%)*

1 +

3.5 + (> 70%)*

1 +

2 + (< 50%)*

1 +

2 + (< 20%)*

< 1

< 1 (0%)*

A172

3 +

3 + (> 55%)*

< 1

2 + (40%)*

< 1

2 + (> 50%)*

< 1

1 + (< 30%)*

< 1

<1 (< 10%)*

< 1

< 1 (0%)*

T98G

< 1

< 1 (0%)*

< 1

< 1 (0%)*

< 1

<1 (0%)*

< 1

<1 (0%)*

< 1

<1 (0%)*

< 1

< 1 (0%)*

  1. GBM cell lines were immunostained for extent of immunostaining for each phenotype. ≤ 1+, negative; 2 + , positive; 3 + , strongly positive; 4 + , more strongly positive. % cells are positive stained cells counted per total 100 cells (*)