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Table 4 Summarized results of in vitro testing for heparin impact on cfDNA assay

From: Autoimmune disorders but not heparin are associated with cell-free fetal DNA test failure

Experiment Sample Heparin Molecule Concentration IU/mL Library prep (nM) Reads (M) Fetal fraction (%) Z-score 21 Z-score 18 Z-score 13
1 NPP No 26.16 20.1 11.7 − 0.78 − 1.55 0.97
1 NPP No 31.01 17.0 10.91 0.92 − 0.85 0.01
1 NPP No 32.29 16.0 10.32 − 0.72 0.00 − 0.42
1 NPP No 30.79 18.9 10.03 − 0.44 − 0.08 − 0.48
1 Entire flow cell No 31.10 18.0 10.20 0.00 0.04 0.01
1 NPP Yes Enoxaparin 0.6 27.05 18.8 10.80 0.36 0.80 − 0.01
1 NPP Yes Enoxaparin 1.2 34.37 18.5 11.10 1.51 0.20 0.24
1 NPP Yes Enoxaparin 2.2.44 23.89 18.7 11.90 0.31 − 0.58 0.45
1 NPP No 22.40 19.9 11.25 − 0.75 − 0.49 − 0.76
1 NPP No 26.28 19.7 10.53 − 0.34 0.22 − 0.20
1 NPP No 29.70 17.0 11.08 − 0.72 − 1.07 − 0.84
1 NPP No 32.84 19.2 11.34 − 0.35 0.04 − 0.46
1 Entire flow cell No 30.98 18.8 9.80 0.02 0.00 0.02
1 NPP Yes Tinzaparin 0.43 27.05 21.6 9.40 − 1.21 0.42 1.49
1 NPP Yes Tinzaparin 0.87 34.37 21.2 9.50 − 0.17 0.04 − 1.43
1 NPP Yes Tinzaparin 1.74 23.89 19.7 10.00 − 0.34 − 0.02 0.24
2 NPP No 38.95 18.6 8.97 0.67 − 0.02 − 2.15
2 NPP No 37.99 18.5 10.32 − 1.17 1.16 − 0.16
2 NPP No 35.89 17.4 11.86 − 1.34 − 0.06 − 0.05
2 NPP No 36.63 18.2 11.85 − 0.10 − 0.72 − 0.73
2 Entire flow cell No 33.40 18.2 10.35 0.06 0.08 0.06
2 NPP Yes Enoxaparin 0.6 37.98 18.9 11.30 0.68 − 0.26 1.25
2 NPP Yes Enoxaparin 1.2 32.51 19.9 10.40 0.15 0.15 − 0.61
2 NPP Yes Enoxaparin 2.2.44 36.92 20.3 11.50 0.35 − 0.37 − 1.03
2 NPP No 22.33 19.8 10.26 0.43 0.66 0.56
2 NPP No 24.38 17.7 10.25 − 0.15 − 0.76 0.51
2 NPP No 26.56 17.5 9.93 − 0.10 0.21 1.21
2 NPP No 28.83 18.0 10.44 0.70 − 0.47 − 0.99
2 Entire flow cell No 29.00 18.2 10.30 − 0.01 0.03 − 0.07
2 NPP Yes Tinzaparin 0.43 32.24 22.2 10.50 0.37 0.92 0.68
2 NPP Yes Tinzaparin 0.87 33.72 19.2 9.50 1.03 0.59 0.56
2 NPP Yes Tinzaparin 1.1.7474 32.06 20.5 10.30 0.19 1.12 0.54
  1. NPP normal plasma pool, entire flow cell: mean data obtained within the same experiments for the 88 samples. Z-score 21: Z-score observed for chromosome 21, Z-score 18: Z-score observed for chromosome 18, Z-score 13: Z-score observed for chromosome 13