Fig. 5

Assessment of total collagen within healing defects. a Representative picro sirius red stained healing defects with ACS (left) and ACS/Talymed (right). BMP2 dose increases from top to bottom. Arrows indicate areas of dense collagen. 10× magnification. b Quantification of total collagen within the defects indicates more collagen in the ACS only defects with collagen area increasing with BMP2 dose. Note that medium BMP2 with ACS only presents the greatest area of collagen. n = 3 sections 40 µm apart from at least four individuals per group ***p ≤ 0.001