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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Matrine attenuates endoplasmic reticulum stress and mitochondrion dysfunction in nonalcoholic fatty liver disease by regulating SERCA pathway

Fig. 6

Effect of Mat on cytosolic Ca2+ level in L02 cells. In the prolonged experiments (a and b), the L02 cells were treated with PA (500 μM), Mat (200, 400, 800 μM) or the combination of PA (500 μM) and Mat (200, 400, 800 μM) for 12 h. Then the cells were incubated with the Fura-3AM fluorescent indicator and measured by fluorescence spectrophotometer. a the fluorescence of the combination of PA and Mat. b the fluorescence of Mat incubated alone. *P < 0.05 and **P < 0.01. In the instantaneous stimulation experiments (ch), the L02 cells were incubated with the Fura-3AM fluorescent indicator and then treated by 2-APB (20 μM), Tg (5 μM) and Mat (200, 400, 800 μM). c Mat-induced instant Ca2+ release of L02 cells. The Ca2+ curves represent an average of three independent experiments. d the combined addition of Mat and Tg (SERCA inhibitor). e reduction of Mat or Tg-induced Ca2+ release by pretreatment with 2-APB (IP3R inhibitor) for 10 min. f the together addition of Mat and 2-APB. g the Ca2+ curves induced by 2-APB after vehicle, Tg or Mat pretreatment for 10 min. h reduction of Mat-induced Ca2+ release by pretreatment with Tg or 2-APB for 12 h

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