Fig. 5

Effect of long-term rIL-18 administration on differentiation, thermogenic-related molecules and the development of lipid accumulation. a, b Il18^+/+ and Il18^−/− mice were treated with rIL-18 twice a week for 12 weeks for long-term treatment from 12 weeks of age, and the protein expression of FGF21, PGC1α, PRDM16 and UCP1 was compared between the groups. c Il18^−/− mice were treated with rIL-18 twice a week for 12 weeks for long-term treatment from 37 weeks of age, and hematoxylin and eosin staining was performed. Scale bars, 50 μm (b, c). (b, n = 3 per group) *p < 0.05. FGF21: fibroblast growth factor 21; PGC1α: peroxisome proliferator-activated receptor γ coactivator 1-α; PRDM16: PR domain containing 16; UCP1: uncoupling protein 1