Fig. 6

Calcipotriol suppressed the activated HSCs-induced tumor progression of heat-treated residual HCC cells in vivo. a, b The changes of Snail, Vimentin, E-cadherin and N-cadherin, PCNA expression in heat-exposed residual HCC cells (MHCC97H, Hep3B, HepG2 and Huh7) cultured with conditioned medium (CM) from HSCs or calcipotriol-treated HSCs were compared. HSCs cells (LX2 and pHSC) were stimulated by the pre-treatment with TGF-β1. c The changes of Snail, Vimentin, E-cadherin and N-cadherin, PCNA expression in heat-treated residual HCC cells were reversed when CM from calcipotriol-treated LX2 cells was supplemented with 100 ng/mL POSTN. d Calcipotriol inhibited tumor growth compared with the control group. The combination therapy of calcipotriol and cisplatin significantly reduced tumor growth. e The expression of POSTN, α-SMA, PCNA, E-Cadherin and cleaved-Caspase-3 was detected by immunohistochemical analysis. Calcipotriol, Cal. **P < 0.01; *P < 0.05