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Table 8 miRNA studies in diabetic kidney disease

From: Genomic approaches in the search for molecular biomarkers in chronic kidney disease

PMID Author Year Sample Patients Ethnics Methodology Main findings
28667184 Cardenas-Gonzalez 2017 Urine Blood Discovery cohort: 10 DKD, 10 HC
Confirmation stage: 58 DKD, 119 HC
Replication stage: 74 DKD, 71 DM, 30 HC
Caucasian 2402 microRNAs Downregulation of miR-2861, miR-1915-3p and miR-4532 in patients with diabetic nephropathy, were (> 10-fold; p < 0.0001)
Downregulation of miR-2861, miR-1915-3p and miR-4532 associated with eGFR (p < 0.01) and interstitial fibrosis/tubular atrophy (p < 0.05)
28923913 Liu 2017 Blood T1DM/T2DM
DKD/HC
  RNA-Seq Downregulation of serum miR-25 in patients with diabetes, both with and without nephropathy (p < 0.001)
26239688 Argyropoulos 2015 Urine 17 T1DM + mAlb 10 T1DM   qRT-PCR, Exiqon miRNA, qPCR panels 1 and 2 The development of microalbuminuria was associated with 18 differentially expressed microRNAs
The predicted targets of these microRNAs map to biological pathways involved in the pathogenesis and progression of diabetic renal disease.
A microRNA signature (miR-105-3p, miR-1972, miR-28-3p, miR-30b-3p, miR-363-3p, miR-424-5p, miR-486-5p, miR-495, miR-548o-3p and for women miR-192-5p, miR-720) achieved high internal validity for microalbuminuria
23358711 Argyropoulos 2013 Urine 10 DM-DKD
10 DM
10 DM + IMA
10 DM + PMA
10 HC
  qRT-PCR
miRNA panel
miRNA-221-3p expression decreased similarly to the comparison of follow up and baseline microalbuminuria samples
miR-589 and miR-323b-5p showed a trend to be increased in the urine of patients with overt nephropathy
Downregulation of miR-323b-5pand upregulation of: miR-122-5p, miR-429 in PMA patients
Appearance of micro-albuminuria was associated with decreased levels of miR-323b-5p and increased urine concentration of miR-429
24223694 Barutta 2013 Urine Exosomes 12 T1DM + mAlb
12 T1DM + nAlb
10 non-diabetics
  Human TaqMan miRNA Array A Differential expression of miR-130a, miR-424, miR-155 and miR-145 in urinary exosomes from microalbuminuric T1DM
Downregulation of miR-155 and miR-424 and upregulation of miR-130a and miR-145 in micro- compared to normoalbuminuric patients
miR-155 and miR-424 could not discriminate between normoalbuminuric diabetic patients and non-diabetic controls
Trend to overexpression of miR-145 and miR-130 in normoalbuminuric diabetic patients compared to controls
23797704 Fiorentino 2013 Tissue 8 DM + DKD
4 HC
  qRT-PCR
TaqMan miRNA reverse transcription kit
Overexpression of miR-21 in kidney biopsies from diabetic patients compared to HC
Downregulation of miR-217 in diabetic subjects compared to HC
20056746 Krupa 2010 Biopsies 22 DKD Caucasian qRT-PCR TaqMan Low Density Array Human MicroRNA Panel 1.0 Two miRNAs differed by more than two-fold between progressors and nonprogressors, and 12 miRNAs differed between late presenters and other biopsies
Late presenters showed the greatest decrease in miR-192 expression
Downregulation of miR-192 correlated with tubulointerstitial fibrosis and low eGFR
  1. DKD: diabetic kidney disease; DM: diabetes mellitus; eGFR:estimated glomerular filtration rate; HC: healthy controls; IMA: intermittent microalbuminuria; mAlb: microalbuminuria; MAlb: macroalbuminuria; nAlb: normoalbuminuria; PMA: persistent microalbuminuria; qRT-PCR: quantitative real-time polymerase chain reaction; RNA: ribonucleic acid; T1DM: type 1 diabetes mellitus; T2DM: type 2 diabetes mellitus