Status of programmed death-ligand 1 expression in sarcomas

Table 1 Summary of the expression status of PD-L1 in human sarcoma cell lines

Cell line	Origin	WB (PD-L1/β-actin ratio)	FACS (%)	ICC (22C3)	ICC (SP142)
A673	Ewing sarcoma	0.06	1.99	N/A	0
GBS-1	UPS	0.23	67.14	N/A	0
HS-RMS-1	Pleomorphic rhabdomyosarcoma	4.73	81.07	3+	3+
HSSYII	Synovial sarcoma	0.16	1.57	0	2+
HT1080	Fibrosarcoma	1.30	92.42	3+	2+
LIPO-224B	DDLPS	0.35	3.42	0	1+
LIPO-246	DDLPS	0.02	3.21	0	0
LIPO-863B	WDLPS	0.63	12.56	0	1+
LP6	DDLPS	1.93	99.6	2+	2+
MG-63	Osteosarcoma	0.02	0.5	0	0
MLS402	Myxoid liposarcoma	0.84	99.6	0	1+
MLS1765	Myxoid liposarcoma	0.55	99.3	0	2+
NMFH-1	Myxofibrosarcoma	0.07	15.34	N/A	0
RH30	Rhabdomyosarcoma	0.61	53.49	0	1+
RH41	Rhabdomyosarcoma	0.01	0.01	0	0
U2-OS	Osteosarcoma	0.42	85.61	0	1+

The staining intensity was graded as 0 (negative), 1+ (weak), 2+ (moderate), and 3+ (strong)
UPS undifferentiated pleomorphic sarcoma, DDLPS dedifferentiated liposarcoma, WDLPS well-differentiated liposarcoma, N/A not available, WB western blot, FACS fluorescence-activated cell sorting, ICC immunocytochemistry

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