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Table 1 Summary of the expression status of PD-L1 in human sarcoma cell lines

From: Status of programmed death-ligand 1 expression in sarcomas

Cell line Origin WB (PD-L1/β-actin ratio) FACS (%) ICC (22C3) ICC (SP142)
A673 Ewing sarcoma 0.06 1.99 N/A 0
GBS-1 UPS 0.23 67.14 N/A 0
HS-RMS-1 Pleomorphic rhabdomyosarcoma 4.73 81.07 3+ 3+
HSSYII Synovial sarcoma 0.16 1.57 0 2+
HT1080 Fibrosarcoma 1.30 92.42 3+ 2+
LIPO-224B DDLPS 0.35 3.42 0 1+
LIPO-246 DDLPS 0.02 3.21 0 0
LIPO-863B WDLPS 0.63 12.56 0 1+
LP6 DDLPS 1.93 99.6 2+ 2+
MG-63 Osteosarcoma 0.02 0.5 0 0
MLS402 Myxoid liposarcoma 0.84 99.6 0 1+
MLS1765 Myxoid liposarcoma 0.55 99.3 0 2+
NMFH-1 Myxofibrosarcoma 0.07 15.34 N/A 0
RH30 Rhabdomyosarcoma 0.61 53.49 0 1+
RH41 Rhabdomyosarcoma 0.01 0.01 0 0
U2-OS Osteosarcoma 0.42 85.61 0 1+
  1. The staining intensity was graded as 0 (negative), 1+ (weak), 2+ (moderate), and 3+ (strong)
  2. UPS undifferentiated pleomorphic sarcoma, DDLPS dedifferentiated liposarcoma, WDLPS well-differentiated liposarcoma, N/A not available, WB western blot, FACS fluorescence-activated cell sorting, ICC immunocytochemistry