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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Status of programmed death-ligand 1 expression in sarcomas

Fig. 1

Expression levels of PD-L1 protein in various human sarcoma cell lines. A Total PD-L1 protein expression was determined by western blotting. The intensity of bands was quantified using ImageJ, and each band was normalized by comparing to levels of β-actin expression. B Cellular surface expression of PD-L1 was quantified by FACS analysis. The intensity of PD-L1 expression in human sarcoma cell lines (a–p) was measured by ICC using PD-L1 22C3 (C) and SP142 (D) antibody clones (×200 magnification). Staining intensity was graded as 0 (negative), 1+ (weak), 2+ (moderate), and 3+ (strong). The proportion of stained cells in the whole region was indicated in parallel (%). a, A673 (ewing sarcoma); b, GBS-1 (UPS); c, HS-RMS-1 (pleomorphic rhabdomyosarcoma); d, HSSYII (synovial sarcoma); e, HT1080 (fibrosarcoma); f, LIPO-224B (DDLPS); g, LIPO-246 (DDLPS); h, LIPO-863B (well-differentiated liposarcoma); i, LP6 (DDLPS); j, MG-63 (osteosarcoma); k, MLS402 (myxoid liposarcoma); l, MLS 1765 (myxoid liposarcoma); m, NMFH-1 (myxofibrosarcoma); n, RH30 (rhabdomyosarcoma); o, RH41 (rhabdomyosarcoma); p, U2-OS (osteosarcoma)

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