Fig. 4

IL-37 inhibited the expression of inflammatory cytokines in PBMCs from patients with sJIA. PBMCs from sJIA patients (n = 46) and HCs (n = 30) were stimulated or not with rhIL-37 (100 ng/ml) for 6 h, cells then cultured with LPS (1 μg/ml) for 4 h, the mRNA levels of IL-6 (a), TNF-α (b), IL-17 (c) and IL-1β (d) were detected by RT-PCR. PBMCs from sJIA patients (n = 46) and HCs (n = 30) were stimulated or not with rhIL-37 (100 ng/ml) for 24 h and then incubated with LPS (1 μg/ml) for 6 h, and IL-6 (e), TNF-α (f), IL-17 (g) and IL-1β (h) levels in supernatant were assessed by ELISA. The data are expressed as mean ± SEM. P values are shown in the graph. NS, no significant. PBMCs, peripheral blood mononuclear cells; TNF-α, Tumor necrosis factor-α