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Fig. 7 | Journal of Translational Medicine

Fig. 7

From: Upregulation of miR-196b-5p attenuates BCG uptake via targeting SOCS3 and activating STAT3 in macrophages from patients with long-term cigarette smoking-related active pulmonary tuberculosis

Fig. 7

miR-196b-5p promoted STAT3 signaling pathway via targeting SOCS3. The relative mRNA expression of SOCS1 (a) and SOCS3 (b) on differentiated U937 cells after exogenously miR-196b-5p, anti-miR-196b-5p transfected were detected by qRT-PCR. Luciferase assay also showed that upregulation of miR-196b-5p attenuated the reporter activity driven by the wildtype 3′-UTRs, but not by the mutant 3′-UTRs of SOCS3 transcripts within miR-196b-5p-binding seed regions in U937 cells (c). Silencing of SOCS3 with SOCS3-RNAi rescued the STAT3 activity repression in miR-196b-5p-silencing U937 cells (d). pSTAT3 were downregulated after overexpressing of SOCS3 in miR-196b-5p-overexpressing U937 cells with SOCS3-LAC (e). Data were presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001

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