Fig. 4

L4.8 immunization augments the pool of polyfunctional CD4 T cells and CD8 T cells. Single cell suspension was prepared from spleen of vaccinated mice and stimulated with L4.8. The polyfunctional CD8 T cells and CD4 T cells were monitored for the intracellular expression of cytokines by flow cytometry. a Contour plots and their b, c bar diagrams represent percent population of cells co-expressing IFN-γ and TNF-α. Similarly, d contour plots and their e, f bar diagrams correspond to cells co-expressing IFN-γ and IL-17A. IFN-γ⁺/IL-17A⁺ CD8 T cells and CD4 T cells were analyzed for the co-expression of CXCR3 and CCR6. g Contour plots and their h, i bar diagrams represent percent population of IFN-γ⁺/IL-17A⁺/CXCR3⁺/CCR6⁺ CD4 T cells and CD8 T cells. Data (mean ± SEM) represented as percent positive cells and are of 2 independent experiments (n = 3 mice/group). *p ≤ 0.05, **p ≤ 0.005, ***p ≤ 0.0005