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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: A lipidated bi-epitope vaccine comprising of MHC-I and MHC-II binder peptides elicits protective CD4 T cell and CD8 T cell immunity against Mycobacterium tuberculosis

Fig. 2

Induction of the protective immune response by L91 and L4.8. The cultures were set as mentioned in the legend to Fig. 1. For L91 group, mice were immunized twice with L91 (20 nmol) at an interval of 1 week. Single cell suspension was prepared from the spleens and lymph nodes obtained from L91 and L4.8 immunized mice. a IFN-γ and b IL-17 were estimated in the culture SNs by ELISA and expressed as ng/ml. c, d Intracellular expression of IFN-γ was assessed by flow cytometry on c CD8 and d CD4 gated T cells. Results are presented as mean ± SEM, (n = 3 mice/group). ab data are representative of 2 independent experiments; c, d pooled splenocytes of three mice from single experiment. **p ≤ 0.005. L91: mice immunized with L91; L4.8: mice immunized with L4.8

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