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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: A lipidated bi-epitope vaccine comprising of MHC-I and MHC-II binder peptides elicits protective CD4 T cell and CD8 T cell immunity against Mycobacterium tuberculosis

Fig. 1

L4.8 elicits secretion of IFN-γ and IL-17 by both CD8 T cells and CD4 T cells. Mice were immunized twice with L4.8 (20 nmol) at an interval of 1 week. After 1 week, animals were sacrificed and cells from the spleen and lymph nodes were pooled and single cell suspension was prepared. The cultures were set and stimulated with L4.8. Control cultures were incubated with F4.8 and Pam2Cys for 72 h. Later, CD8 and CD4 gated T cells were analyzed for the intracellular expression of IFN-γ and IL-17A. a Flow cytometry histogram and b, c bar diagrams indicate IFN-γ expressing CD8 T cells and CD4 T cells. Likewise, d histogram and e, f bar diagrams represent IL-17A expressing CD8 T cells and CD4 T cells. Data represented as mean ± SEM are percent population from 2 independent experiments (n = 3 mice/group). *p ≤ 0.05, **p ≤ 0.005, ***p ≤ 0.0005, ****p ≤ 0.0001

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