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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Establishment of urinary exosome-like vesicles isolation protocol for FHHNC patients and evaluation of different exosomal RNA extraction methods

Fig. 2

uEVs characterization. a Representative cryo-TEM images of all the uEVs pellets obtained from different isolation procedures. Scale bar is 100 nm except in P7 which is 200 nm. b NTA results of the uEVs recovered after DTT treatment. P2 showed higher peaks than P3 that might correspond to impurities observed by cryo-TEM. c NTA profiles of the uEVs pellets obtained from the five different uEVs isolation protocols. In all cases, the mode size falls within the expected size range. Even though the highest peak is observed in P5, the associated cryo-TEM image suggests that the peak might correspond to impurities. d Bar graph showing uEVs concentration by NTA. Because of P5 impurities, NTA quantification may not be accurate. P4 and P6 contained the highest amount of uEVs. Data is expressed as the mean ± SD. e Equal volumes of uEVs (20 µL) were loaded for immunoblotting against Alix and TSG101. The strongest signal was obtained in P4, followed by P6. Even though a strong band of Alix is observed in P5, this is slightly above the expected weight. f Immunoblotting of uEVs isolated from 300 mL of FHHNC urine, using procedure P4, showed a strong intensity of bands corresponding to Alix and TSG101, indicating uEVs presence and its absence in the supernatant

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