Fig. 5
From: Identification of serum β-catenin as a biomarker in patients with HBV-related liver diseases
Akt/GSK3β signaling is responsible for elevated β-catenin expression in HBV-infected hepatic cells. a Real-time PCR analysis of β-catenin gene in L02 cells transfected with and without pcDNA3.1-HBV (1.3 and 1.1) or its control pcDNA3.1 for 24 h. b Western blot analysis of p-GSK3β and p-Akt expression in L02 cells transfected with and without pcDNA3.1-HBV (1.3 and 1.1) or its control pcDNA3.1 for 48 h. c, d Western blot analysis of p-GSK3β, p-Akt (c) and β-catenin (d) expression in L02 cells transfected with and without pcDNA3.1-HBV (1.3) or its control pcDNA3.1 followed by treatment with LY294002 (20 μM) for 48 h. e Immunofluorescence staining for β-catenin expression in L02 cells transfected with and without pcDNA3.1-HBV (1.3) or its control pcDNA3.1 followed by treatment with LY294002 (20 μM) for 48 h. White scale bars = 50 µm