Fig. 4
From: Identification of serum β-catenin as a biomarker in patients with HBV-related liver diseases
Increased β-catenin expression in HBV-infected hepatic cells. a Western blot analysis of β-catenin expression in hepatic L02 cells transfected with and without pcDNA3.1-HBV (1.3 and 1.1) or its control pcDNA3.1 for 48 h. β-actin served as a loading control. b Immunofluorescence staining for β-catenin in L02 cells transfected with and without pcDNA3.1-HBV (1.3 and 1.1) or its control pcDNA3.1 for 48 h. White scale bars = 50 µm. c ELISA assay for β-catenin expression in L02 cells transfected with and without pcDNA3.1-HBV (1.3 and 1.1) or its control pcDNA3.1 for 48 h. d LDH assay for LDH from L02 cells transfected with and without pcDNA3.1-HBV (1.3 and 1.1) or its control pcDNA3.1 for 48 h. e Flow cytometry analysis for apoptotic L02 cells transfected with and without pcDNA3.1-HBV (1.3 and 1.1) or its control pcDNA3.1 for 72 h. f Apoptosis index for L02 cells with different treatment. Ns no statistical significance