Fig. 3

a Nucleus fragmentation. APOCC alone (control), pre-incubated with H1 or pre-incubated with H1+ a blocking antibody for IL-8 (bAB IL-8) were treated with Taxol (20 µM). Confocal microscopy was performed on the cells stained with DAPI. Pictures are representative of the nucleus in the well. Scale bar: 5 µm. b The relative quantification of apoptosis genes was performed by real-time qPCR on APOCC after different treatment (H0, H1, bAB IL-8). Relative transcript levels are represented as the log10 of ratios between the two subpopulations of their 2^−ΔΔCp real-time PCR values. c APOCC cells pre-incubated or not with H1 with or without bAB IL-8 for 24 h were treated with Taxol (20 µM). Caspase and cleaved Caspase 3 and 9 were assessed by western blot. Induced apoptosis on APOCC using an anti-Fas receptor (CD95) monoclonal antibody (mAb) was used as a positive control