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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Surgical peritoneal stress creates a pro-metastatic niche promoting resistance to apoptosis via IL-8

Fig. 2

a Cytokine array. Cytokine arrays were performed on H0 and H1 of five patients (numbered 1–5 on the figure). Each column in the cluster graphics represents one sample and each line a cytokine. The intensity ranges from green (less expressed) to red (more expressed). b Pixel density of selected cytokines. The pixel density of the most expressed cytokines in H1 are represented on the histogram normalized with the H0 pixel density. c MTT assay. APOCC were pre-treated for 24 h with human recombinant cytokines (CD54, IL-6, IL-8, Serpin E1, Rantes, C5/C5a or G-CSF) prior to Taxol treatment (20 µM). After 48 h a MTT assay was performed. The histogram represents the mean OD MTT. d Cell viability. APOCC were pre-incubated with H0 or H1 prior Taxol (20 µM) treatment (left panel). APOCC pre-incubated with H0 were also incubated with specific blocking antibodies for IL-6, IL-8, Rantes or G-CSF prior Taxol (20 µM) treatment (right panel). The percentage of live cells (blue), dead cells (red), apoptotic cells (purple) and debris (green) are represented in the histogram on the right

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