Fig. 5

INC280 and MetMab inhibit HGF induced tube formation in HUVEC cells. a Representative images of HUVEC cell tube formation   treated with either INC280 (1 µM) or MetMab (1 µM). HUVEC cells were cultured in a 24-well plate coated with 50 μl Matrigel for the treatments as indicated. Triplicates were used for each treatment. After 16 h, cells were imaged by phase-contrast microscopy. Five images were taken randomly from each well. Tube formation before and after treatment are shown in upper and lower panel, respectively. b Quantification of tube-like structures as described above in a. The number of tubes is calculated as mean ± SD of 15 images that are measured by Image J. Fold change refers to the average number of tubes of each treatment group as compared to HGF stimulation alone group. Vertical bar refers to standard deviation. *Compared with the HGF stimulation control group, inhibition was found to be statistically significant (student t test, p < 0.05). c INC280 (1 µM) and MetMab (1 µM) inhibit phosphorylation of MET, MAPK/AKT and Src/p85 signaling pathway proteins in HUVEC cells as shown by western blot