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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Differential responses of MET activations to MET kinase inhibitor and neutralizing antibody

Fig. 3

INC280 induces p53BP1 and γH2AX foci-formation in MKN45 and MHCC97H cells. MKN45 and MHCC97H cells were treated with vehicle or INC280 (1 µM) for 18 h and fixed for IF staining with γH2AX (green) or 53BP1 (red) antibodies and DAPI (blue) for nuclear staining. Triplicates were used for each concentration. Each co-localization of γH2AX and 53BP1 (yellow) is counted as one foci-formation at a DNA double-strand break. For each treatment at least 100 nuclei were counted based on fluorescence images (60×) to quantify foci formation. a Representative images showing the nuclei of cells with p53BP1 (red) and γH2AX (green) localization and foci formation (yellow) in MKN45 and MHCC97H cells. b The percentage of cells with 0, 1, and 1+ foci after vehicle or INC280 (1 µM) treatment for 18 h. *Compared with the control group, the increase of foci formation in the INC280-treated group was statistically significant (Chi square test, p < 0.05). c INC280 (1 µM) treatment for 24 h up-regulates the ATM/Chk1 pathway in MHCC97H cells and this up-regulation can be inhibited by the specific ATM inhibitor KU00019 as shown by western blot analysis. d Combination of INC280 and KU00019 treatment for 72 h results in a higher inhibitory efficacy in MHCC97H cells as measured by the CellTiter-Glo assay. Triplicates were used for each concentration. Data represents the mean ± SD for each treatment. Vertical bar represents the standard deviation

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