Fig. 3

Osteogenic differentiation potential of MSC from MDS patients with high and low BM serum HA levels. a After 21 days in osteogenic medium, representative micrographs of Alizarin Red S staining of MDS patients with high and low BM serum HA levels are shown. Red staining highlights deposition of mineralized extracellular matrix by differentiated MSC. Magnification: 10×. b Differences with regard to osteogenic differentiation were quantified by the OD value. c Comparion of mRNA expression of osteogenic differentiation related genes (RUNX-2, BSP, ALP, COL-1, OPN, OCN) between MDS patients with high and low BM serum HA levels