Fig. 7
From: RIP3 dependent NLRP3 inflammasome activation is implicated in acute lung injury in mice
RIP3 silencing inhibited LPS-induced NLRP3 inflammasome activation in THP-1 cells. THP-1 cells were transfected with 100 nmol siRNA or scramble siRNA and cultured for 48–72 h before stimulation. a Representative western blot bands showed the protein expression of RIP3, p-MLKL, MLKL, NLRP3, Caspase-1, Caspase-1 p20 and GAPDH. b Quantitative data showed protein expressions of RIP3, p-MLKL, NLRP3, and caspase-1 p20 normalized to the values of RIP3, MLKL, GAPDH and caspase-1 respectively. Each bar represents mean ± SEM (n = 3). *p < 0.05, **p < 0.01 versus PBS group; #p < 0.05, ##p < 0.01 versus LPS + ATP group