Fig. 6
From: RIP3 dependent NLRP3 inflammasome activation is implicated in acute lung injury in mice
GSK872 inhibits LPS-induced NLRP3 activation by reducing RIP1–RIP3 and RIP3–NLRP3 interaction in THP-1 cells. a RIP1–RIP3 and RIP3–NLRP3 interactions in THP-1 cells were detected by Duolink® In situ detection reagents and visualized by a fluorescence microscope. Signals were shown in green (red arrows indicated RIP1 and RIP3 interaction, yellow arrows indicated RIP3 and NLRP3 interaction) and the nuclei in blue. b RIP1–RIP3 and RIP3–NLRP3 interactions in THP-1 cells were further detected by immunoprecipitation study. RIP3 complexes isolated by immunoprecipitation from THP-1 cells were analyzed for RIP1, RIP3 and NLRP3 expression by western blot