Fig. 3

GSK872 reduced active caspase-1 level in lung interstitial macrophages (IMs) and CD45⁺I-A⁺CD11b^hiCD24⁺ monocyte–macrophages/dendritic cells (M–M/DCs) of LPS-induced ALI mice. a Identification of CD45⁺SiglecF⁺CD11c⁺CD11b^lo alveolar macrophages, CD45⁺I-A⁺CD11b⁺CD24^lo IMs, CD45⁺I-A⁺CD11b^hiCD24⁺ monocyte–macrophages/dendritic cells (M–M/DCs) and CD45⁺CD103⁺ CD11b^lo dendritic cells (CD103⁺ DCs) in the lung tissues of sham mouse. b IMs and M–M/DCs in Sham (PBS control), LPS and GSK872 (LPS + GSK872 treatment) groups. c Cell count percentage of IMs and M–M/DCs to the total cell counts determined by FACS. d Median fluorescence intensity (MFI) of active caspase-1 in IMs and M–M/DCs determined by FACS. Each bar represents mean ± SEM (n = 4). *p < 0.05 versus sham mice; ^#p < 0.05 versus LPS-induced lung injury mice