Fig. 1

hBMMSCs cultured in atmospheric and hypoxic conditions display similar identity. Sample-matched hBMMSCs cultured in atmospheric or hypoxic conditions for 3 passages from the time of isolation were tested for the expression of surface markers characteristic of MSCs (CD90, CD105, CD73 and CD49b) and the absence of expression of hematopoietic markers (CD34 and CD45) by flow cytometry. a, b Representative dot plots of atmospheric cultured (a) and hypoxic cultured (b) hBMMSCs are presented. Positive for CD90, CD105, CD73 and CD49b whereas quadrant Q3-1 display cells that are double negative for CD34 and CD45. c, d Quantification of the percentage of cells that are CD34/CD45 double negative (c) and CD90/CD105 double positive (d) in hBMMSCs cultured in atmospheric and hypoxic conditions. Data represent mean ± SD, n = 3 independent sample-matched human BMMSCs. Two-tailed paired Student’s t-test: non-significant (NS) p value > 0.05