Fig. 6

Antibiotic treatment does not change antibacterial protein expression as well as ROS production of intestine and peroxynitrite production as well as phagocytic activity of AMs in MyD88^−/− mice. a Antibiotic treatment decrease DCFDA levels of the intestinal mucosa in WT mice but not in MyD88^−/− mice. FOS feeding after antibiotic treatment did not change DCFDA levels of the intestinal mucosa in MyD88^−/− mice as compared with the antibiotic group. The levels of reactive oxygen species in the intestinal mucosa were analyzed by DCFDA fluorescent dye. DCF is detected by fluorescence spectroscopy with excitation and emission spectra of 495 and 529 nm, respectively. Data are presented as mean ± SEM. b Antibiotic treatment did not change the peroxynitrite production of AMs in MyD88^−/− mice as compared with the control group. FOS treatment after antibiotic did not change the peroxynitrite production of AMs in MyD88^−/− mice as compared with the antibiotic group. Data are presented as mean ± SEM. c Antibiotic treatment did not change the PA phagocytic activity of AMs in MyD88^−/− mice as compared with the control group. FOS treatment after antibiotic did not change PA phagocytic activity of AMs in MyD88^−/− mice as compared with the antibiotic group. Data are presented as mean ± SEM. d Antibiotic treatment did not change RELMβ and Reg3β of the intestinal mucosa in MyD88^−/− mice. FOS treatment after antibiotic did not change RELMβ and Reg3β in MyD88^−/− mice. Data are presented as mean ± SEM. e Antibiotic treatment did not change NF-κB DNA binding activity of the intestinal mucosa in MyD88^−/− mice as compared with the control group. FOS treatment after antibiotic did not change NF-κB DNA binding activity in MyD88^−/− mice as compared with the antibiotic group. Data are presented as mean ± SEM; DCF, 2ʹ7ʹ-dichlorofluorescein; FOS, fructo-oligosaccharides